生物学论文_糖酵解途径相关基因与植物乳杆菌生

文章目录

1 Materials and Methods

1.1 Materials and reagents

    1.1.1 Bacterial strains,cells,and culture conditions

    1.1.2 Construction of plasmids and bacterial strains

    1.1.3 Other materials and reagents

1.2 Instruments and equipments

1.3 Methods

    1.3.1 Real-time PCR

    1.3.2 Growth curves

    1.3.3 Stress resistance assay

    1.3.4 Biofilm formation

    1.3.5 Adhesion assay

    1.3.6 Bioinformatics

1.4 Statistical analysis

2 Results and Analysis

2.1 The transcription levels of the pgm,fba and gap genes in the recombinant strains

2.2 The overexpression of the pgm,fba and gap genes affected the growth of LR-1

2.3 The overexpression of the pgm,fba and gap genes affected the stress resistances of LR-1

2.4 The overexpression of the pgm,fba and gap genes affected the biofilm formation of LR-1

2.5 The overexpression of the pgm,fba and gap genes affected the adhesion ability of LR-1

2.6 The overexpression of the pgm,fba and gap genes affected the transcription levels of other genes in LR-1

2.7 Bioinformatics analyses

3 Discussion

4 Conclusions

文章摘要:研究pgm、fba、gap过表达对植物乳杆菌（Lactobacillus plantarum）LR-1的抗逆性、黏附和生物被膜形成的影响。结果表明,pgm、fba基因的过表达可提高植物乳杆菌LR-1的耐热性、耐酸性和抗胆盐性,这两个基因的过表达促进了该菌株生物被膜的形成和黏附。gap基因的转录水平对植物乳杆菌LR-1的抗逆性、生物被膜形成和黏附没有显著影响。pgm、fba、gap的上调提高了该菌株tuf、luxS的转录水平。这种增加在过表达pgm、fba的重组菌株中比在过表达gap的重组菌株中高。此外,生物信息学分析表明,pgm、fba可与多条途径相关的基因相关联。本研究揭示糖酵解途径相关基因在调节植物乳杆菌的抗逆性、黏附和生物被膜形成中的新作用。

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项目基金:《植物生理学报》 网址: http://www.zwslxbzz.cn/qikandaodu/2021/1202/808.html